Is Gus a reporter gene?
Is Gus a reporter gene?
The reporter gene was the uidA gene of Escherichia coli that encodes the enzyme β-glucuronidase (GUS). Since then, the uidA gene (commonly referred to as the gus gene) has become one of the most widely used reporter genes in plant molecular biology.
What is the purpose of the GUS reporter gene?
The functional GUS gene produces blue coloration in plants upon integration into the plant genome. Because of the ease it provides to analyze the gene expression (as no expensive equipment is needed), GUS gene is surely plant biotechnologist’s first choice as a reporter gene.
What are GUS reporter lines?
The GUS reporter system (GUS: β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique.
What is reporter gene in biology?
Reporter genes are those genes that when introduced into target cells (e.g., brain tissues, cancer, and circulating white cells) produce a protein receptor or enzyme that binds, transports, or traps a subsequently injected imaging probe.
What is a GFP reporter?
Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells.
How GUS gene is used in plant transformation?
Agrobacterium tumefaciens is a commonly used tool for transforming dicotyledonous plants. A portable intron, derived from a plant intron, was introduced into the beta-glucuronidase (GUS) gene. In transgenic plants containing this chimaeric gene the intron is spliced efficiently, giving rise to GUS enzymatic activity.
How do you test for luciferase activity?
To measure luciferase activity of the cell lysates, you will need a multiwell plate or a tube containing cell lysates and a luminometer. This apparatus detects the photon emission produced from the luciferase reaction and the unit of measurement comes out as relative light units (RLU).
How do you create a gene reporter?
To activate reporter genes, they can be expressed constitutively, where they are directly attached to the gene of interest to create a gene fusion. This method is an example of using cis-acting elements where the two genes are under the same promoter elements and are transcribed into a single messenger RNA molecule.
What is cat gene?
The CAT gene provides instructions for making pieces (subunits) of an enzyme called catalase. Four identical subunits, each attached (bound) to an iron-containing molecule called a heme group, form the functional enzyme.