What does B27 supplement contain?

April 12, 2020 Off By idswater

What does B27 supplement contain?

B-27 Serum-Free Supplement (50X) liquid

Components Concentration (mg/L) mM
Vitamins
Glutathione (reduced) confidential n/a
L-Carnitine HCl confidential n/a
Linoleic Acid confidential n/a

How do you defrost a B27?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Does B27 contain insulin?

B27 contains numerous hormones and growth factors to avoid cell-death of cultivated neurons. Its classic formulae (ref 17504–044) notably contains insulin, at a final concentration of 58 nM, according to our determinations.

What is N2 medium?

NDiff N2 medium is a proprietary, completely defined, serum-free cell-culture supplement for the derivation, maintenance, expansion, and/or differentiation of human and mouse stem cells and tissue-derived neural stem (NS) cells.

What is B27 for?

B27 is a supplement which is added to serum free media to promote growth and proliferation of cells without differentiation (no FBS). It is true it is used for neuronal growth but it is not specific for it.

What does having HLA-B27 positive mean?

A positive HLA-B27 test means that the person tested is at increased risk of developing certain autoimmune diseases.

What is a HLA-B27 blood test?

HLA-B27 is a blood test to look for a protein that is found on the surface of white blood cells. The protein is called human leukocyte antigen B27 (HLA-B27). Human leukocyte antigens (HLAs) are proteins that help the body’s immune system tell the difference between its own cells and foreign, harmful substances.

Is GlutaMAX the same as L glutamine?

Gibco GlutaMAX supplement is an improved cell culture supplement that can be used as a direct substitute for L-glutamine in your cell culture media. GlutaMAX supplement is a dipeptide, L-alanine-L-glutamine, which is more stable in aqueous solutions and does not spontaneously degrade.

What is in Neurobasal media?

Neurobasal™ Media are basal media formulated to meet the neuronal cells special requirements. They allow for long-term maintenance of the normal phenotype and growth of neuronal cells, and maintain pure populations of neuronal cells without the need of an astrocyte feeder layer. Calcium Chloride (CaCl2) (anhyd.)

What does N2 supplement do?

Description. N-2 Supplement is a chemically-defined, serum-free supplement based on Bottenstein’s N-1 formulation. It is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system.

What are the symptoms of HLA-B27?

joint pain. stiffness or swelling of your spine, neck, or chest. inflammation of your joints or urethra accompanied by skin lesions. recurring inflammation in your eye.