What is the function of elongation factor 1?

June 8, 2019 Off By idswater

What is the function of elongation factor 1?

Elongation factor 1-alpha 1 (eEF1a1) is a protein that in humans is encoded by the EEF1A1 gene. This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome.

What is ef1α?

EF1A (also known as EF-1alpha or EF-Tu) is a G-protein. It forms a ternary complex of EF1A-GTP-aminoacyltRNA. The binding of aminoacyl-tRNA stimulates GTP hydrolysis by EF1A, causing a conformational change in EF1A that causes EF1A-GDP to detach from the ribosome, leaving the aminoacyl-tRNA attached at the A-site.

What is the function of eEF2?

Eukaryotic elongation factor 2 (eEF2) is a GTP-binding protein that mediates the translocation of peptidyl-tRNA from the A site to the P site on the ribosome (Moldave, 1985).

Where is EF-Tu found?

EF-Tu has been found in large quantities in the cytoskeletons of bacteria, co-localizing underneath the cell membrane with MreB, a cytoskeletal element that maintains cell shape.

What is eEF1A protein?

Eukaryotic translation elongation factor 1A (eEF1A) is one of the most abundant protein synthesis factors. eEF1A is responsible for the delivery of all aminoacyl-tRNAs to the ribosome, aside from initiator and selenocysteine tRNAs.

What is a U6 promoter?

U6 is a type III RNA polymerase III promoter commonly used for driving small hairpin RNA (shRNA) expression in vector-based RNAi. In the design and construction of viral vectors, multiple transcription units may be arranged in close proximity in a space-limited vector.

Is diphtheria A cytotoxin?

Diphtheria toxin is an exotoxin secreted by Corynebacterium, the pathogenic bacterium that causes diphtheria. The toxin gene is encoded by a prophage called corynephage β.

What is ADP ribosylation of elongation factor 2?

Eukaryotic elongation factor 2 is the target of a class of bacterial mono-ADP-ribosyltransferase toxins which include the prototype, DT, exotoxin A from P. aeruginosa, and cholix toxin from V. Exposure of eukaryotic cells to these toxins leads to inhibition of protein synthesis and cell growth.

What is the primary function of EF G and EF-Tu?

An excellent example is the mechanism of the elongation cycle in protein synthesis, that uses the elongation factor Tu (EF-Tu) to deliver aminoacyl-tRNA (aa-tRNA) to the mRNA-programmed ribosome, and the elongation factor G (EF-G) to help the translocation of the tRNA–mRNA complex on the ribosome.

What is the full form of EF TS?

EF-Ts (elongation factor thermo stable) is one of the prokaryotic elongation factors. It is found in human mitochrondria as TSFM. It is similar to eukaryotic EF-1B.

How strong is U6 promoter?

U6 promoter is constitutively active in a variety of cell types and maintains relatively high activity by providing approximately 4×105 transcripts per cell (29).